e coli p aeruginosa shuttle vector Search Results


e coli p aeruginosa shuttle vector  (ATCC)
93
ATCC e coli p aeruginosa shuttle vector
Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. <t>aeruginosa</t> and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.
E Coli P Aeruginosa Shuttle Vector, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+p+aeruginosa+shuttle+vector/pmc07980316-259-19-27?v=ATCC
Average 93 stars, based on 1 article reviews
e coli p aeruginosa shuttle vector - by Bioz Stars, 2026-07
93/100 stars
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mucolipin 3 mcoln3 human shrna plasmid kit  (OriGene)
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MCOLN3 Human 4 unique 29mer shRNA constructs in lentiviral GFP vector
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mucolipin 1 crispr/cas9 ko plasmid  (Santa Cruz Biotechnology)
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CRISPR/Cas9 KO Plasmids consists of mucolipin 1-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
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mucolipin1-pegfp c3 (plasmid #62960)  (Addgene inc)
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mucolipin1 d471-472k-phcred c1 (plasmid #62961)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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mucolipin 1 shrna plasmid  (Santa Cruz Biotechnology)
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Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of mucolipin 1 gene silencing results, individual duplex components or plasmids are also available upon request.
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mucolipin 1 mcoln1 human shrna plasmid kit  (OriGene)
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MCOLN1 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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mucolipin 3 hdr plasmid  (Santa Cruz Biotechnology)
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CRISPR/Cas9 KO Plasmids consists of mucolipin 3-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
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mucolipin 2 double nickase plasmid  (Santa Cruz Biotechnology)
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CRISPR/Cas9 KO Plasmids consists of mucolipin 2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
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mucolipin 2 crispr activation plasmid  (Santa Cruz Biotechnology)
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CRISPR/Cas9 KO Plasmids consists of mucolipin 2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
   Buy from Supplier
mucolipin 2 shrna plasmid  (Santa Cruz Biotechnology)
N/A
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of mucolipin 2 gene silencing results, individual duplex components or plasmids are also available upon request.
   Buy from Supplier
mucolipin 3 crispr activation plasmid  (Santa Cruz Biotechnology)
N/A
CRISPR/Cas9 KO Plasmids consists of mucolipin 3-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break
   Buy from Supplier

Image Search Results


Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. aeruginosa and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.

Journal: ACS chemical biology

Article Title: Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics

doi: 10.1021/acschembio.9b00875

Figure Lengend Snippet: Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. aeruginosa and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.

Article Snippet: The fluorescent-reporter systems were excised from pUC57 with restriction enzymes Bam HI and Pst I and ligated into an E. coli - P. aeruginosa shuttle vector, pCN61 (ATCC).

Techniques: Plasmid Preparation, Fluorescence, Selection, Construct, Labeling, Transformation Assay

Correlation of the β-lactamase and fluorescence assays. (A) Nitrocefin hydrolysis by AmpC β-lactamase shifts λmax from 390 nm (yellow) to 486 nm (red). (B) Nitrocefin assay performed for P. aeruginosa wild-type and dacB::Tn is plotted as the slope of the absorbance at 486 nm over time for two concentrations of FOX (1/8 MIC and 1/4 MIC) and no antibiotic. (C) Fluorescent response expressed as relative fluorescence (A.U.) of P. aeruginosa wild-type and dacB::Tn under the same experimental conditions as used for the nitrocefin assay. The error bars represent the standard deviation of three biological replicates.

Journal: ACS chemical biology

Article Title: Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics

doi: 10.1021/acschembio.9b00875

Figure Lengend Snippet: Correlation of the β-lactamase and fluorescence assays. (A) Nitrocefin hydrolysis by AmpC β-lactamase shifts λmax from 390 nm (yellow) to 486 nm (red). (B) Nitrocefin assay performed for P. aeruginosa wild-type and dacB::Tn is plotted as the slope of the absorbance at 486 nm over time for two concentrations of FOX (1/8 MIC and 1/4 MIC) and no antibiotic. (C) Fluorescent response expressed as relative fluorescence (A.U.) of P. aeruginosa wild-type and dacB::Tn under the same experimental conditions as used for the nitrocefin assay. The error bars represent the standard deviation of three biological replicates.

Article Snippet: The fluorescent-reporter systems were excised from pUC57 with restriction enzymes Bam HI and Pst I and ligated into an E. coli - P. aeruginosa shuttle vector, pCN61 (ATCC).

Techniques: Fluorescence, Standard Deviation

GFP-labeled P. aeruginosa harboring pCN61_AmpRtdT was imaged on a swarm plate in proximity of a second bacterium, either (A) P. mesacidophila, (B) B. licheniformis, (C) E. coli, or (D) M. xanthus. Each panel depicts the bacteria after 15 h of growth. P. aeruginosa is to the left of each plate, while the second bacterium is to the right (see plate panels). The white arrow (top plate) points to a representative site where the two bacteria encounter one another. The second column from left shows bright-field images of comingled bacteria, where the strains meet, as uniform continuous lawns. The GFP panel shows the green fluorescence displayed by P. aeruginosa in this lawn. The black voids are the locations of the second bacterium in this same lawn. The RFP panel shows the red-fluorescent signal from P. aeruginosa that results from contact with an antibiotic-producer strain. Red fluorescence is seen for P. mesacidophila and B. lichemiformis as antibiotic producers. No red fluorescence is seen for E. coli and M. xanthus (not antibiotic producers). The far-right column merges the green and red fluorescent images. A 10-μm black scale bar is given in the bright-field image of panel A.

Journal: ACS chemical biology

Article Title: Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics

doi: 10.1021/acschembio.9b00875

Figure Lengend Snippet: GFP-labeled P. aeruginosa harboring pCN61_AmpRtdT was imaged on a swarm plate in proximity of a second bacterium, either (A) P. mesacidophila, (B) B. licheniformis, (C) E. coli, or (D) M. xanthus. Each panel depicts the bacteria after 15 h of growth. P. aeruginosa is to the left of each plate, while the second bacterium is to the right (see plate panels). The white arrow (top plate) points to a representative site where the two bacteria encounter one another. The second column from left shows bright-field images of comingled bacteria, where the strains meet, as uniform continuous lawns. The GFP panel shows the green fluorescence displayed by P. aeruginosa in this lawn. The black voids are the locations of the second bacterium in this same lawn. The RFP panel shows the red-fluorescent signal from P. aeruginosa that results from contact with an antibiotic-producer strain. Red fluorescence is seen for P. mesacidophila and B. lichemiformis as antibiotic producers. No red fluorescence is seen for E. coli and M. xanthus (not antibiotic producers). The far-right column merges the green and red fluorescent images. A 10-μm black scale bar is given in the bright-field image of panel A.

Article Snippet: The fluorescent-reporter systems were excised from pUC57 with restriction enzymes Bam HI and Pst I and ligated into an E. coli - P. aeruginosa shuttle vector, pCN61 (ATCC).

Techniques: Labeling, Fluorescence